anti hp1γ Search Results


hp1γ  (Merck & Co)
90
Merck & Co hp1γ
Nuclear distribution pattern of heterochromatin protein 1 (HP1) isoforms in non-irradiated and γ-irradiated human cervix adenocarcinoma (HeLa) cells. Morphology of ( a ) HP1α (see GFP-tagged HP1α, green), ( b ) HP1β (see GFP-tagged HP1β, green), and ( c ) <t>HP1γ</t> (see GFP-tagged HP1γ, green) was studied by laser scanning confocal microscopy. The HP1-positive foci (green) were analyzed in the vicinity of fibrillarin-positive regions of nucleoli (red fluorescence signals). DAPI staining (blue) was used for visualization of nuclei. Scale bars show 5 µm. ( d ) Analysis of the number of HP1α-, HP1β-, and HP1γ-positive foci in non-irradiated and γ-irradiated (5 Gy) cells. Cells were fixed for analysis 2 h after γ-irradiation. Fifty cell nuclei per sample were studied. Asterisk in panel (d) shows significantly different result.
Hp1γ, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hp1γ/product/Merck & Co
Average 90 stars, based on 1 article reviews
hp1γ - by Bioz Stars, 2026-03
90/100 stars
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anti-hp1γ clone 2mod-1g6as  (Euromedex)
90
Euromedex anti-hp1γ clone 2mod-1g6as
Nuclear distribution pattern of heterochromatin protein 1 (HP1) isoforms in non-irradiated and γ-irradiated human cervix adenocarcinoma (HeLa) cells. Morphology of ( a ) HP1α (see GFP-tagged HP1α, green), ( b ) HP1β (see GFP-tagged HP1β, green), and ( c ) <t>HP1γ</t> (see GFP-tagged HP1γ, green) was studied by laser scanning confocal microscopy. The HP1-positive foci (green) were analyzed in the vicinity of fibrillarin-positive regions of nucleoli (red fluorescence signals). DAPI staining (blue) was used for visualization of nuclei. Scale bars show 5 µm. ( d ) Analysis of the number of HP1α-, HP1β-, and HP1γ-positive foci in non-irradiated and γ-irradiated (5 Gy) cells. Cells were fixed for analysis 2 h after γ-irradiation. Fifty cell nuclei per sample were studied. Asterisk in panel (d) shows significantly different result.
Anti Hp1γ Clone 2mod 1g6as, supplied by Euromedex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-hp1γ clone 2mod-1g6as/product/Euromedex
Average 90 stars, based on 1 article reviews
anti-hp1γ clone 2mod-1g6as - by Bioz Stars, 2026-03
90/100 stars
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rabbit anti-hp1γ  (Huabio Inc)
90
Huabio Inc rabbit anti-hp1γ
Nuclear distribution pattern of heterochromatin protein 1 (HP1) isoforms in non-irradiated and γ-irradiated human cervix adenocarcinoma (HeLa) cells. Morphology of ( a ) HP1α (see GFP-tagged HP1α, green), ( b ) HP1β (see GFP-tagged HP1β, green), and ( c ) <t>HP1γ</t> (see GFP-tagged HP1γ, green) was studied by laser scanning confocal microscopy. The HP1-positive foci (green) were analyzed in the vicinity of fibrillarin-positive regions of nucleoli (red fluorescence signals). DAPI staining (blue) was used for visualization of nuclei. Scale bars show 5 µm. ( d ) Analysis of the number of HP1α-, HP1β-, and HP1γ-positive foci in non-irradiated and γ-irradiated (5 Gy) cells. Cells were fixed for analysis 2 h after γ-irradiation. Fifty cell nuclei per sample were studied. Asterisk in panel (d) shows significantly different result.
Rabbit Anti Hp1γ, supplied by Huabio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti-hp1γ/product/Huabio Inc
Average 90 stars, based on 1 article reviews
rabbit anti-hp1γ - by Bioz Stars, 2026-03
90/100 stars
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anti-hp1γ  (ABclonal Biotechnology)
90
ABclonal Biotechnology anti-hp1γ
Nuclear distribution pattern of heterochromatin protein 1 (HP1) isoforms in non-irradiated and γ-irradiated human cervix adenocarcinoma (HeLa) cells. Morphology of ( a ) HP1α (see GFP-tagged HP1α, green), ( b ) HP1β (see GFP-tagged HP1β, green), and ( c ) <t>HP1γ</t> (see GFP-tagged HP1γ, green) was studied by laser scanning confocal microscopy. The HP1-positive foci (green) were analyzed in the vicinity of fibrillarin-positive regions of nucleoli (red fluorescence signals). DAPI staining (blue) was used for visualization of nuclei. Scale bars show 5 µm. ( d ) Analysis of the number of HP1α-, HP1β-, and HP1γ-positive foci in non-irradiated and γ-irradiated (5 Gy) cells. Cells were fixed for analysis 2 h after γ-irradiation. Fifty cell nuclei per sample were studied. Asterisk in panel (d) shows significantly different result.
Anti Hp1γ, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-hp1γ/product/ABclonal Biotechnology
Average 90 stars, based on 1 article reviews
anti-hp1γ - by Bioz Stars, 2026-03
90/100 stars
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anti-hp1γ  (GeneTex)
90
GeneTex anti-hp1γ
Nuclear distribution pattern of heterochromatin protein 1 (HP1) isoforms in non-irradiated and γ-irradiated human cervix adenocarcinoma (HeLa) cells. Morphology of ( a ) HP1α (see GFP-tagged HP1α, green), ( b ) HP1β (see GFP-tagged HP1β, green), and ( c ) <t>HP1γ</t> (see GFP-tagged HP1γ, green) was studied by laser scanning confocal microscopy. The HP1-positive foci (green) were analyzed in the vicinity of fibrillarin-positive regions of nucleoli (red fluorescence signals). DAPI staining (blue) was used for visualization of nuclei. Scale bars show 5 µm. ( d ) Analysis of the number of HP1α-, HP1β-, and HP1γ-positive foci in non-irradiated and γ-irradiated (5 Gy) cells. Cells were fixed for analysis 2 h after γ-irradiation. Fifty cell nuclei per sample were studied. Asterisk in panel (d) shows significantly different result.
Anti Hp1γ, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-hp1γ/product/GeneTex
Average 90 stars, based on 1 article reviews
anti-hp1γ - by Bioz Stars, 2026-03
90/100 stars
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anti-heterochromatin protein 1 γ (hp1 γ ) (clone 42s2)  (Upstate Biotechnology Inc)
90
Upstate Biotechnology Inc anti-heterochromatin protein 1 γ (hp1 γ ) (clone 42s2)
Nuclear distribution pattern of heterochromatin protein 1 (HP1) isoforms in non-irradiated and γ-irradiated human cervix adenocarcinoma (HeLa) cells. Morphology of ( a ) HP1α (see GFP-tagged HP1α, green), ( b ) HP1β (see GFP-tagged HP1β, green), and ( c ) <t>HP1γ</t> (see GFP-tagged HP1γ, green) was studied by laser scanning confocal microscopy. The HP1-positive foci (green) were analyzed in the vicinity of fibrillarin-positive regions of nucleoli (red fluorescence signals). DAPI staining (blue) was used for visualization of nuclei. Scale bars show 5 µm. ( d ) Analysis of the number of HP1α-, HP1β-, and HP1γ-positive foci in non-irradiated and γ-irradiated (5 Gy) cells. Cells were fixed for analysis 2 h after γ-irradiation. Fifty cell nuclei per sample were studied. Asterisk in panel (d) shows significantly different result.
Anti Heterochromatin Protein 1 γ (Hp1 γ ) (Clone 42s2), supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-heterochromatin protein 1 γ (hp1 γ ) (clone 42s2)/product/Upstate Biotechnology Inc
Average 90 stars, based on 1 article reviews
anti-heterochromatin protein 1 γ (hp1 γ ) (clone 42s2) - by Bioz Stars, 2026-03
90/100 stars
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Image Search Results


Nuclear distribution pattern of heterochromatin protein 1 (HP1) isoforms in non-irradiated and γ-irradiated human cervix adenocarcinoma (HeLa) cells. Morphology of ( a ) HP1α (see GFP-tagged HP1α, green), ( b ) HP1β (see GFP-tagged HP1β, green), and ( c ) HP1γ (see GFP-tagged HP1γ, green) was studied by laser scanning confocal microscopy. The HP1-positive foci (green) were analyzed in the vicinity of fibrillarin-positive regions of nucleoli (red fluorescence signals). DAPI staining (blue) was used for visualization of nuclei. Scale bars show 5 µm. ( d ) Analysis of the number of HP1α-, HP1β-, and HP1γ-positive foci in non-irradiated and γ-irradiated (5 Gy) cells. Cells were fixed for analysis 2 h after γ-irradiation. Fifty cell nuclei per sample were studied. Asterisk in panel (d) shows significantly different result.

Journal: Cells

Article Title: DNA Damage Changes Distribution Pattern and Levels of HP1 Protein Isoforms in the Nucleolus and Increases Phosphorylation of HP1β-Ser88

doi: 10.3390/cells8091097

Figure Lengend Snippet: Nuclear distribution pattern of heterochromatin protein 1 (HP1) isoforms in non-irradiated and γ-irradiated human cervix adenocarcinoma (HeLa) cells. Morphology of ( a ) HP1α (see GFP-tagged HP1α, green), ( b ) HP1β (see GFP-tagged HP1β, green), and ( c ) HP1γ (see GFP-tagged HP1γ, green) was studied by laser scanning confocal microscopy. The HP1-positive foci (green) were analyzed in the vicinity of fibrillarin-positive regions of nucleoli (red fluorescence signals). DAPI staining (blue) was used for visualization of nuclei. Scale bars show 5 µm. ( d ) Analysis of the number of HP1α-, HP1β-, and HP1γ-positive foci in non-irradiated and γ-irradiated (5 Gy) cells. Cells were fixed for analysis 2 h after γ-irradiation. Fifty cell nuclei per sample were studied. Asterisk in panel (d) shows significantly different result.

Article Snippet: For immunofluorescence analysis, we used the following antibodies: HP1α (#05-689, Merck, Czech Republic), HP1β (#MAB3448, Merck, Czech Republic), HP1γ (#05-690, Merck, Czech Republic), KAP1 (#ab10483, Abcam, UK), anti-histone H2AX phosphorylated at the S139 position (γH2AX; #ab2893, Abcam, UK), 53BP1 (#MAB3802, Merck, Czech Republic), and fibrillarin (#ab4566, Abcam, UK).

Techniques: Irradiation, Confocal Microscopy, Fluorescence, Staining

ChIP-polymerase chain reaction (ChIP-PCR) analysis of HP1α, HP1β, HP1γ, KAP1 abundance in ribosomal genes. ( a ) The analysis was performed in non-treated human cervix adenocarcinoma (HeLa) cells, and cells irradiated by 5 Gy of γ-rays. Cells were harvested 2 h after γ-irradiation. The highest density in rDNA encoding 28S rRNA and rDNA promoter region was observed for HP1γ. ( b ) Quantification of fragment densities, shown in panel ( a ), was done by ImageJ software. White asterisk shows statistically significant difference, shown by Student’s t-test at p ≤ 0.05.

Journal: Cells

Article Title: DNA Damage Changes Distribution Pattern and Levels of HP1 Protein Isoforms in the Nucleolus and Increases Phosphorylation of HP1β-Ser88

doi: 10.3390/cells8091097

Figure Lengend Snippet: ChIP-polymerase chain reaction (ChIP-PCR) analysis of HP1α, HP1β, HP1γ, KAP1 abundance in ribosomal genes. ( a ) The analysis was performed in non-treated human cervix adenocarcinoma (HeLa) cells, and cells irradiated by 5 Gy of γ-rays. Cells were harvested 2 h after γ-irradiation. The highest density in rDNA encoding 28S rRNA and rDNA promoter region was observed for HP1γ. ( b ) Quantification of fragment densities, shown in panel ( a ), was done by ImageJ software. White asterisk shows statistically significant difference, shown by Student’s t-test at p ≤ 0.05.

Article Snippet: For immunofluorescence analysis, we used the following antibodies: HP1α (#05-689, Merck, Czech Republic), HP1β (#MAB3448, Merck, Czech Republic), HP1γ (#05-690, Merck, Czech Republic), KAP1 (#ab10483, Abcam, UK), anti-histone H2AX phosphorylated at the S139 position (γH2AX; #ab2893, Abcam, UK), 53BP1 (#MAB3802, Merck, Czech Republic), and fibrillarin (#ab4566, Abcam, UK).

Techniques: Polymerase Chain Reaction, Irradiation, Software

Fluorescence Lifetime Imaging-Forster Resonance Energy Transfer (FLIM-FRET) analysis of potential interaction between heterochromatin protein 1 (HP1) protein isoforms and the KAP1 protein. ( a ) Analysis of a GFP-tagged HP1α-KAP1, b] GFP-tagged HP1β-KAP1, and c] GFP-tagged HP1γ-KAP1. ( b ) An example of a nuclear distribution of HP1γ and the KAP1 protein. HP1γ-positive regions of nucleoli (green) were absent of KAP1. Abbreviation Nu means nucleoli; arrows show HP1γ foci absent of KAP1 and red frames show HP1γ foci colocalizing with KAP1. ( c ) Example of STED analysis showing the location of KAP1 inside the cell nucleoli decorated by the HP1 isoforms (see HP1β in white frame). ( d ) FLIM-FRET analysis of a] GFP-tagged HP1β ΔCD/Alexa 594-KAP1, b] GFP-tagged HP1β ΔCSD/ Alexa 594-KAP1, and c] GFP-tagged HP1β ΔHinge/Alexa 594-KAP1. E [%] means FLIM-FRET efficiency. Scale bars represent 4 µm.

Journal: Cells

Article Title: DNA Damage Changes Distribution Pattern and Levels of HP1 Protein Isoforms in the Nucleolus and Increases Phosphorylation of HP1β-Ser88

doi: 10.3390/cells8091097

Figure Lengend Snippet: Fluorescence Lifetime Imaging-Forster Resonance Energy Transfer (FLIM-FRET) analysis of potential interaction between heterochromatin protein 1 (HP1) protein isoforms and the KAP1 protein. ( a ) Analysis of a GFP-tagged HP1α-KAP1, b] GFP-tagged HP1β-KAP1, and c] GFP-tagged HP1γ-KAP1. ( b ) An example of a nuclear distribution of HP1γ and the KAP1 protein. HP1γ-positive regions of nucleoli (green) were absent of KAP1. Abbreviation Nu means nucleoli; arrows show HP1γ foci absent of KAP1 and red frames show HP1γ foci colocalizing with KAP1. ( c ) Example of STED analysis showing the location of KAP1 inside the cell nucleoli decorated by the HP1 isoforms (see HP1β in white frame). ( d ) FLIM-FRET analysis of a] GFP-tagged HP1β ΔCD/Alexa 594-KAP1, b] GFP-tagged HP1β ΔCSD/ Alexa 594-KAP1, and c] GFP-tagged HP1β ΔHinge/Alexa 594-KAP1. E [%] means FLIM-FRET efficiency. Scale bars represent 4 µm.

Article Snippet: For immunofluorescence analysis, we used the following antibodies: HP1α (#05-689, Merck, Czech Republic), HP1β (#MAB3448, Merck, Czech Republic), HP1γ (#05-690, Merck, Czech Republic), KAP1 (#ab10483, Abcam, UK), anti-histone H2AX phosphorylated at the S139 position (γH2AX; #ab2893, Abcam, UK), 53BP1 (#MAB3802, Merck, Czech Republic), and fibrillarin (#ab4566, Abcam, UK).

Techniques: Fluorescence, Imaging, Förster Resonance Energy Transfer

Immunoprecipitation analysis of potential interaction between HP1α, HP1β, and HP1γ. Studies of following possible interactions: ( a ) HP1α-HP1α, HP1α-HP1β, HP1α-HP1γ; HP1α-KAP1; ( b ) HP1β-HP1α, HP1β-HP1β, HP1β-HP1γ; HP1β-KAP1; ( c ) HP1γ-HP1α, HP1γ-HP1β, HP1γ-HP1γ, HP1γ-KAP1. Protein-protein interaction was studied in non-treated HeLa cells, and cells treated with SAHA (15 µM) and irradiated by γ-rays (5 Gy). Cells were harvested for analysis 2 h the treatment. ( d ) Western blot analysis of following proteins: HP1α, HP1β, HP1γ, KAP1, normalized to the total protein levels and α-tubulin, and H3K9ac, H3S10ph, γH2AX, normalized to the level of total histone H3. ( e ) Data from large panel ( d ) were quantified by ImageJ software. Levels of HP1 isoforms were normalized to the level of α-tubulin, and γH2AX or H3S10ph levels were normalized to the level of total histone H3. Profiles of HP1α, HP1β, HP1γ, KAP1, H3S10ph, and γH2AX were studied in (no. 1) non-irradiated HeLa cells, γ-irradiated Hela cells by (no. 2) 5 Gy of γ-rays/harvested after 2h; (no. 3) 2 Gy/harvested after 10 min; (no. 4) 2 Gy/harvested after 30 min; (no. 5) combination of SAHA treatment with 2 Gy/harvested after 10 min; and (no. 6) combination of SAHA treatment with 2 Gy/harvested after 30 min. A small panel in ( d ) is showing western blot data for non-treated HeLa cells, and cells irradiated by 5 Gy of γ-rays or SAHA (15 µM) treated HeLa cells.

Journal: Cells

Article Title: DNA Damage Changes Distribution Pattern and Levels of HP1 Protein Isoforms in the Nucleolus and Increases Phosphorylation of HP1β-Ser88

doi: 10.3390/cells8091097

Figure Lengend Snippet: Immunoprecipitation analysis of potential interaction between HP1α, HP1β, and HP1γ. Studies of following possible interactions: ( a ) HP1α-HP1α, HP1α-HP1β, HP1α-HP1γ; HP1α-KAP1; ( b ) HP1β-HP1α, HP1β-HP1β, HP1β-HP1γ; HP1β-KAP1; ( c ) HP1γ-HP1α, HP1γ-HP1β, HP1γ-HP1γ, HP1γ-KAP1. Protein-protein interaction was studied in non-treated HeLa cells, and cells treated with SAHA (15 µM) and irradiated by γ-rays (5 Gy). Cells were harvested for analysis 2 h the treatment. ( d ) Western blot analysis of following proteins: HP1α, HP1β, HP1γ, KAP1, normalized to the total protein levels and α-tubulin, and H3K9ac, H3S10ph, γH2AX, normalized to the level of total histone H3. ( e ) Data from large panel ( d ) were quantified by ImageJ software. Levels of HP1 isoforms were normalized to the level of α-tubulin, and γH2AX or H3S10ph levels were normalized to the level of total histone H3. Profiles of HP1α, HP1β, HP1γ, KAP1, H3S10ph, and γH2AX were studied in (no. 1) non-irradiated HeLa cells, γ-irradiated Hela cells by (no. 2) 5 Gy of γ-rays/harvested after 2h; (no. 3) 2 Gy/harvested after 10 min; (no. 4) 2 Gy/harvested after 30 min; (no. 5) combination of SAHA treatment with 2 Gy/harvested after 10 min; and (no. 6) combination of SAHA treatment with 2 Gy/harvested after 30 min. A small panel in ( d ) is showing western blot data for non-treated HeLa cells, and cells irradiated by 5 Gy of γ-rays or SAHA (15 µM) treated HeLa cells.

Article Snippet: For immunofluorescence analysis, we used the following antibodies: HP1α (#05-689, Merck, Czech Republic), HP1β (#MAB3448, Merck, Czech Republic), HP1γ (#05-690, Merck, Czech Republic), KAP1 (#ab10483, Abcam, UK), anti-histone H2AX phosphorylated at the S139 position (γH2AX; #ab2893, Abcam, UK), 53BP1 (#MAB3802, Merck, Czech Republic), and fibrillarin (#ab4566, Abcam, UK).

Techniques: Immunoprecipitation, Irradiation, Western Blot, Software

Fluorescence lifetime imaging-Forster resonance energy transfer (FLIM-FRET) analysis of potential interaction between HP1α, HP1β, and HP1γ. Following interactions were studied: ( a ) a) GFP-tagged HP1α/mCherry-HP1β, b) GFP-tagged HP1β/ mCherry-HP1β, c] mCherry-HP1β/GFP-tagged HP1γ. E (%) means FLIM-FRET efficiency. FLIM-FRET analysis of ( b ) a) GFP-tagged HP1β ΔCD/mCherry-HP1β, b) GFP-tagged HP1β ΔCSD/mCherry-HP1β, and c ) GFP-tagged HP1β ΔHinge/mCherry-HP1β. E (%) means FLIM-FRET efficiency. Scale bars represent 2 µm.

Journal: Cells

Article Title: DNA Damage Changes Distribution Pattern and Levels of HP1 Protein Isoforms in the Nucleolus and Increases Phosphorylation of HP1β-Ser88

doi: 10.3390/cells8091097

Figure Lengend Snippet: Fluorescence lifetime imaging-Forster resonance energy transfer (FLIM-FRET) analysis of potential interaction between HP1α, HP1β, and HP1γ. Following interactions were studied: ( a ) a) GFP-tagged HP1α/mCherry-HP1β, b) GFP-tagged HP1β/ mCherry-HP1β, c] mCherry-HP1β/GFP-tagged HP1γ. E (%) means FLIM-FRET efficiency. FLIM-FRET analysis of ( b ) a) GFP-tagged HP1β ΔCD/mCherry-HP1β, b) GFP-tagged HP1β ΔCSD/mCherry-HP1β, and c ) GFP-tagged HP1β ΔHinge/mCherry-HP1β. E (%) means FLIM-FRET efficiency. Scale bars represent 2 µm.

Article Snippet: For immunofluorescence analysis, we used the following antibodies: HP1α (#05-689, Merck, Czech Republic), HP1β (#MAB3448, Merck, Czech Republic), HP1γ (#05-690, Merck, Czech Republic), KAP1 (#ab10483, Abcam, UK), anti-histone H2AX phosphorylated at the S139 position (γH2AX; #ab2893, Abcam, UK), 53BP1 (#MAB3802, Merck, Czech Republic), and fibrillarin (#ab4566, Abcam, UK).

Techniques: Fluorescence, Imaging, Förster Resonance Energy Transfer